Part:BBa_K1361001:Design

Curli Fiber generator under the control of T7 promoter with a relatively weak expression of CsgA,C

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 646
  Illegal NheI site found at 669
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.

Source

CsgB and CsgAC were collected by PCR from genomic DNA of E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers.